http://informativejournals.com/journal/index.php/tjpls <p align="justify"><strong>Tropical Journal of Pharmaceutical and Life Sciences-TJPLS Journal (ISSN : 2349-9818) </strong>is a Scholarly Double-Blind Peer-reviewed Open Access international journal which promotes the original work in field of pharmaceutical and life sciences. Necessity is the mother of invention and invention arises from researches. Therefore promoting researchers is way to solving the problems of humanity. Pharmacy is the field which is for serving the mankind and to relieve it from suffering. TJPLS accepts original research papers as well as review articles in the field of Pharmaceutical and Life Sciences.</p> Jadoun Science Publishing Group India en-US Tropical Journal of Pharmaceutical and Life Sciences 2349-9818 http://informativejournals.com/journal/index.php/tjpls/article/view/213 <p>Nutraceuticals are dietary supplements that contain bioactive compounds, which can provide health benefits beyond basic nutrition. The use of nutraceuticals has gained popularity in recent years due to their potential to prevent and manage various diseases. This review aims to provide an overview of the current state of knowledge on nutraceuticals, their types, and their potential health benefits (Kalra, 2003). We also discuss the challenges and limitations associated with the use of nutraceuticals and highlight future directions for research.</p> Rambir Singh Copyright (c) 2025 Rambir Singh https://creativecommons.org/licenses/by-nc/4.0 2025-12-07 2025-12-07 12 5 01 11 10.61280/tjpls.v12i5.213 http://informativejournals.com/journal/index.php/tjpls/article/view/195 <p><em>Moringa oleifera</em> seed oil (MSO) is widely recognized for its medicinal properties, particularly its antimicrobial potential. This study investigates the influence of extraction methods hot extraction (MOH), cold extraction (MOC), and a control on the antibacterial and antifungal activities of MSO. Antibacterial assays revealed that the MOC extract exhibited the broadest and most potent activity, notably against <em>Escherichia coli</em> (15.5 mm inhibition zone) and methicillin-resistant <em>Staphylococcus aureus</em> (MRSA) (23.75 mm), outperforming MOH and the control. Conversely, MOH and the control showed more potent activity against <em>Enterococcus faecalis</em>, with inhibition zones of 14.5 mm and 24.5 mm, respectively. Overall, MSO demonstrated greater efficacy against Gram-positive bacteria (14.5–24.5 mm) than Gram-negative strains (13–25 mm), with statistically significant differences observed for <em>E. coli</em> and <em>MRSA</em> across extraction methods. Antifungal evaluations showed minimum inhibitory concentrations (MIC) ranging from 12.5 to 50 mg/mL, depending on the fungal isolate. While MOC and the control exhibited similar antifungal profiles, MOH displayed variable activity, reduced against <em>Aspergillus flavus</em> (MIC = 50 mg/mL) and enhanced against Trichoderma sp. These findings underscore the critical role of the extraction technique in modulating the antimicrobial and antifungal efficacy of Moringa seed oil, supporting its potential application as a natural therapeutic agent.</p> Issoufou Amadou Massaoudou Mahamane Ibrahim Halilou Amadou Mahamadou Rabiou Moudi Aboubacar Copyright (c) 2025 Issoufou Amadou, Massaoudou Mahamane, Ibrahim Halilou Amadou, Mahamadou Rabiou Moudi Aboubacar https://creativecommons.org/licenses/by-nc/4.0 2025-10-27 2025-10-27 12 5 12 28 10.61280/tjpls.v12i5.195