In-Vitro Analysis of Ethanol Extract of Saponaria Officinalis for Antiurolithiatic Activity

Authors

  • Ms. Sowmya B A Assistant Professor, Department of Pharmacology, East West College of Pharmacy, Bengaluru, Karnataka, India.
  • Dr. Satish Pavuluri Associate Professor, Institute of Pharmacy, Shri Jagdishprasad Jhabarmal Tibrewala University, Churela, Jhunjhunu, Rajasthan.

DOI:

https://doi.org/10.61280/tjpls.v10i5.147

Keywords:

Urolithiasis, Nucleation, Aggregation, Calcium Chloride, Sodium Oxalate, Saponaria officinalis

Abstract

The goal of the current study was to assess Saponaria officinalis ethanol extracts' in-vitro anti-urolithiatic effectiveness as a potential natural therapy to combat adverse effects brought on by the use of contemporary synthetic medications. S. officinalis extracted with ethanol was put to the test for its ability to treat urolithiasis utilizing in vitro studies (nucleation, aggregation assays). This extract was evaluated and contrasted with the reference medication cystone at increasing concentrations of 200, 400, 600, 800, and 1000μg/ml. The extract's ability to suppress the growth of calcium oxalate crystals in preformed forms was evaluated. The results of the S. officinalis extract on the nucleation assay were significant and showed considerable inhibition 64.2%±0.5 compared to standard cystone drug 67.7%±0.8 at 1000μg/ml concentration. Additionally, the ethanol extract of S. officinalis showed significant inhibition in the aggregation assay with a difference of 74.2%±0.08 compared to cystone 76.7%±0.09 at 1000μg/ml concentration. At a dose level of 1000μg/ml, the ethanol extract of S. officinalis possesses anti-urolithiatic potentials in vitro. It is possible to further exploit the ethanol extract of S. officinalis as a potential anti-urolithiasis medication.

Downloads

Download data is not yet available.

References

Harper JD, Cunitz BW, Dunmire B, Lee FC, Sorensen MD, Hsi RS, et al. First in human clinical trial of ultrasonic propulsion of kidney stones. J Urol. 2016; 195 (4):956-64.

Tyagi S, Patel C, Patel J, Tarun PS. Review on Kidney Stones. Journal of Biomedical and Pharmaceutical Research. 2012; 1(3):06-09.

Havagiray R, Chitme SA, Jain SK, Monika S. Herbal treatment for urinary stones. IJPSR 2010; 1:1-10.

Ratkalkar VN, Kleinman JG. Mechanisms of Stone Formation. Clinical reviews in Bone and Mineral Metabolism. 2011; 9(3-4):187-197.

Bahmani M, Baharvand-Ahmadi B, Tajeddini P, Rafieian-Kopaei M, Naghdi N. Identification of medicinal plants for the treatment of kidney and urinary stones. Journal of Renal Injury Prevention. 2016; 5(3):129-133.

Stamatelou KK, Francis ME, Jones CA, Nyberg Jr LM, Curhan GC. Time trends in reported prevalence of kidney stones in the United States: 1976-1994. Kidney International. 2003; 63(5):1817-1823.

Chaudhary A, Singla S, Tandon C. In vitro evaluation of Terminalia arjuna on calcium phosphate and calcium oxalate crystallization. Indian Journal of Pharmaceutical Sciences. 2010; 72(3):340.

Patel KM, Samir KS. Evaluation of Antiurolithiatic Activity of Lawsonia inermis Linn in Rats. International Journal of Pharmaceutical Science and Nanotechnology. 2017; 10(1):3728-3735.

Aggarwal A, Tandon S, Singla SK, Tandon C. Reduction of oxalate-induced renal tubular epithelial (NRK-52E) cell injury and inhibition of calcium oxalate crystallisation in vitro by aqueous extract of Achyranthes aspera. International Journal of Green Pharmacy. 2010; 4(3):480-489.

Bhattarai S, Chaudhary RP, Quave CL, Taylor RSL. The use of medicinal plants in the trans-Himalayan arid zone of Mustang district, Nepal. Journal of Ethnobiology and Ethnomedicine. 2010; 6:4-14.

Kumaran MS, Patki PS. Evaluation of an Ayurvedic formulation (Cystone), in urolithiasis: A double blind, placebo-controlled study. European Journal of Integrative Medicine. 2011; 3(1):23-28.

Brardi S, Imperiali P, Cevenini G, Verdacchi T, Ponchietti R. Effects of the association of potassium citrate and Agropyrum repens in renal stone treatment: results of a prospective randomized comparison with potassium citrate. Archivio Italiano di Urologia e Andrologia. 2012; 84(2):61-67.

Patel PK, Patel MA, Vyas BA, Shah DR, Gandhi TR. Antiurolithiatic activity of saponin rich fraction from the fruits of Solanum xanthocarpum Schrad. And Wendl. (Solanaceae) against ethylene glycol induced urolithiasis in rats. J Ethnopharmacol. 2012; 144 (1), 160-70.

Sampath Kumar KP, Bhowmik D, Duraivel S, Umadevi M. Traditional and medicinal uses of banana. J Pharmacogn. Phytochem. 2012; 1:53.

Vekilov PG. Nucleation. Cryst Growth Des. 2010; 10(12):5007–19.

Karamakar D, Patki P. Evaluation of efficacy and safety of a herbal formulation cystone in the management of urolithiasis: meta-analysis of 50 clinical studies. Int J Altern Med. 2013; 8(1):1–10.

Sharma D, Dey YN, Sikarwar I, Sijoria R, Wanjari MM, Jadhav AD. In vitro study of aqueous leaf extract of Chenopodium album for inhibition of calcium oxalate and brushite crystallization. Egypt J Basic Appl Sci. 2016; 3(2): 164–71.

Chaudhary D, Paudel S, Rana RM, Timsina S, Malla KP, Giri PM, Neupane BP. Inhibition of calcium oxalate crystallization in vitro by methanolic leaf extract of Murraya koenigii (L.) Spreng. Int J Herb Med. 2018; 6(2):13–5.

Onyema CT, Ofor CE, Okudo VC, Ogbuagu AS. Phytochemical and antimicrobial analysis of banana pseudo stem (Musa acuminata). J Pharma Res Int. 2016:1–9.

Fouada A, Yamina S, Nait MA, Mohammed B, Abdlekrim R. In vitro and in vivo antilithiatic effect of saponin rich fraction isolated from Herniaria hirsuta. J Bras Nefrol. 2006; 28: 199–203.

Sivaraman D. Evaluation of anti- urolithiasis potential of Indian medicinal herbs Ficus hispida, Morinda tinctoria and Sapindus emarginatus by struvite crystal growth inhibition assay. Int J ChemTech Res. 2017; 10 (10):436–48.

Published

23-09-2023

How to Cite

Ms. Sowmya B A, and Dr. Satish Pavuluri. “In-Vitro Analysis of Ethanol Extract of Saponaria Officinalis for Antiurolithiatic Activity”. Tropical Journal of Pharmaceutical and Life Sciences, vol. 10, no. 5, Sept. 2023, pp. 57-62, doi:10.61280/tjpls.v10i5.147.
Statistics
178 Views | Downloads

Issue

Section

Research Article