Effects of Organic Moringa Seed Oil Extraction Methods on Antibacterial and Antifungal Properties: Extraction Dependent on the Antimicrobial Efficacy of Organic Moringa Seed Oil

Issoufou Amadou; Massaoudou Mahamane; Ibrahim Halilou Amadou; Mahamadou Rabiou  Moudi Aboubacar

doi:10.61280/tjpls.v12i5.195

Issoufou Amadou Dan Dicko Dankoulodo University of Maradi, Niger
Massaoudou Mahamane School of Post Graduate (SISE), Dan Dicko Dankoulodo University of Maradi, Niger
Ibrahim Halilou Amadou André Salifou University, Department of Biological Sciences, Faculty of Science and Technology, Lab-EGB2S, ZURID, Zinder, Niger
Mahamadou Rabiou Moudi Aboubacar Africa Centre of Excellence for Neglected Tropical Disease and Forensic Biotechnology, Ahmadu Bello University Zaria, Kaduna State, Nigeria

DOI: https://doi.org/10.61280/tjpls.v12i5.195

Keywords:

Antibacterial activity, Moringa, Bioactive compounds, Water based extraction

Abstract

Moringa oleifera seed oil (MSO) is widely recognized for its medicinal properties, particularly its antimicrobial potential. This study investigates the influence of extraction methods hot extraction (MOH), cold extraction (MOC), and a control on the antibacterial and antifungal activities of MSO. Antibacterial assays revealed that the MOC extract exhibited the broadest and most potent activity, notably against Escherichia coli (15.5 mm inhibition zone) and methicillin-resistant Staphylococcus aureus (MRSA) (23.75 mm), outperforming MOH and the control. Conversely, MOH and the control showed more potent activity against Enterococcus faecalis, with inhibition zones of 14.5 mm and 24.5 mm, respectively. Overall, MSO demonstrated greater efficacy against Gram-positive bacteria (14.5–24.5 mm) than Gram-negative strains (13–25 mm), with statistically significant differences observed for E. coli and MRSA across extraction methods. Antifungal evaluations showed minimum inhibitory concentrations (MIC) ranging from 12.5 to 50 mg/mL, depending on the fungal isolate. While MOC and the control exhibited similar antifungal profiles, MOH displayed variable activity, reduced against Aspergillus flavus (MIC = 50 mg/mL) and enhanced against Trichoderma sp. These findings underscore the critical role of the extraction technique in modulating the antimicrobial and antifungal efficacy of Moringa seed oil, supporting its potential application as a natural therapeutic agent.

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