ANTIOXIDANT POTENTIAL OF VARIOUS EXTRACTS OF COCCINA INDICA, SIDA CORDATA, MEDICAGO SATIVA AND WEDELIA TRILOBATA USING DPPH METHOD

Mantry Piyush; Dr. Jat K. Rakesh

doi:10.61280/tjpls.v9i6.111

Authors

Mantry Piyush Research Scholar, Shri Jagdishprasad Jhabarmal Tibrewala University, Chudela, Raj. 333001
Dr. Jat K. Rakesh Principal, Department of pharmacy, Shri Jagdishprasad Jhabarmal Tibrewala University, Chudela, Raj. 333001

DOI:

https://doi.org/10.61280/tjpls.v9i6.111

Keywords:

DPPH, Antioxidant, Coccina indica, Sida cordata, Medicago sativa, Wedelia trilobata

Abstract

Background - As an element safeguarding the body's health, antioxidants are crucial. Antioxidants may lower your chance of developing chronic diseases including cancer, Liver and heart disease, according to scientific research. The majority of the antioxidant substances in a regular diet come from plant sources and fall under several groups of substances with a wide range of physical and chemical characteristics.

Objective - To evaluate the Antioxidant activity of the various extracts of Coccina indica, Sida cordata, Medicago sativa and . Wedelia trilobata leaves using 2, 2-Diphenyl-1-Picrylhydrazyl (DPPH) Method.

Methods –

The selected plant components are crushed into a fine powder, and this powder is then sieved through a specific number of sieve 40 to create a homogenous powder. Additionally, this powder has been tested in accordance with WHO guidelines. It was discovered that all of the measured parameters fell inside the defined ranges. All of the plant parts were extracted using pet ether, chloroform, ethanol, and aqueous after physicochemical evaluation.

Results –

In this study Antioxidant activity was performed by DPPH (1, 1diphenyl-2-picrylhydrazyl) radical scavenging method for different extracts of leaves of of Coccinaindica, Sidacordata, Medicago sativa and Wedeliatrilobata. plant species which showed that alcoholic extract of leaves of this plant on higher concentration possess better antioxidant potential when compare to reference standard ascorbic acid. They exhibited strong antioxidant DPPH radical scavenging activity with IC50 value of9.3 and 24.8 μg/ml for ascorbic acid and alcoholic leaves extract respectively. The absorbance for reducing power was found to be 0.0390, 0.0989 for ascorbic acid and alcoholic leaves extract respectively. The strongest antioxidant activity of ethanol extract could be due to the presence of flavonoids and phenols.

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