• Mantry Piyush Research Scholar, Shri Jagdishprasad Jhabarmal Tibrewala University, Chudela, Raj. 333001
  • Dr. Jat K. Rakesh Principal, Department of pharmacy, Shri Jagdishprasad Jhabarmal Tibrewala University, Chudela, Raj. 333001



DPPH, Antioxidant, Coccina indica, Sida cordata, Medicago sativa, Wedelia trilobata


Background - As an element safeguarding the body's health, antioxidants are crucial. Antioxidants may lower your chance of developing chronic diseases including cancer, Liver and heart disease, according to scientific research. The majority of the antioxidant substances in a regular diet come from plant sources and fall under several groups of substances with a wide range of physical and chemical characteristics.

Objective - To evaluate the Antioxidant activity of the various extracts of Coccina indica, Sida cordata, Medicago sativa and . Wedelia trilobata leaves using 2, 2-Diphenyl-1-Picrylhydrazyl (DPPH) Method.

Methods –

The selected plant components are crushed into a fine powder, and this powder is then sieved through a specific number of sieve 40 to create a homogenous powder. Additionally, this powder has been tested in accordance with WHO guidelines. It was discovered that all of the measured parameters fell inside the defined ranges. All of the plant parts were extracted using pet ether, chloroform, ethanol, and aqueous after physicochemical evaluation.

Results –

In this study Antioxidant activity was performed by DPPH (1, 1diphenyl-2-picrylhydrazyl) radical scavenging method for different extracts of leaves of of Coccinaindica, Sidacordata, Medicago sativa and Wedeliatrilobata. plant species which showed that alcoholic extract of leaves of this plant on higher concentration possess better antioxidant potential when compare to reference standard ascorbic acid. They exhibited strong antioxidant DPPH radical scavenging activity with IC50 value of9.3 and 24.8 μg/ml for ascorbic acid and alcoholic leaves extract respectively. The absorbance for reducing power was found to be 0.0390, 0.0989 for ascorbic acid and alcoholic leaves extract respectively. The strongest antioxidant activity of ethanol extract could be due to the presence of flavonoids and phenols.


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Bijekar SR, Gayatri M. Ethanomedicinal properties of Euphorbiaceae family-a comprehensive review. Int J Phytomed. 2014;6(2):144–156.

Kipkore W, Wanjohi B, Rono H, Kigen G. A study of the medicinal plants used by the Marakwet Community in Kenya. J EthnobiolEthnomed. 2014;10(1):22. doi:10.1186/1746-4269-10-24

Enyew A, Asfaw Z, Kelbessa E, Nagappan R. Ethnobotanical study of traditional medicinal plants in and around Fiche District, Central Ethiopia. Curr Res J Biol Sci. 2014;6(4):154–167. doi:10.19026/crjbs.6.5515

Matu EN. ClutiaabyssinicaJaub. &Spach. In: Schmelzer GH, Gurib-Fakim A, editors. PROTA (Plant Resources of Tropical Africa/RessourcesVégétales De l’AfriqueTropicale). Wageningen, Netherlands; 2008. Available from: Accessed January 2020.

de Boer HJ, Kool A, Broberg A, Mziray WR, Hedberg I, Levenfors JJ. Anti-fungal and anti-bacterial activity of some herbal remedies from Tanzania. J Ethnopharmacol. 2005;96(3):461–469. doi:10.1016/j.jep.2004.09.035

Kigen G, Some F, Kibosia J. Ethnomedicinal plants traditionally used by the keiyo community in ElgeyoMarakwet County, Kenya. J BiodiversBiopros Dev. 2014;1:3.

Andemariam SW. Legislative Regulation of Traditional Medicinal Knowledge in Eritrea via-a-vis Eritrea’s Commitments under the Convention on Biological Diversity: issues and Alternatives. Law Env’t Dev J. 2010;6:130.

Teklay A, Abera B, Giday M. An ethnobotanical study of medicinal plants used in KilteAwulaelo District, Tigray Region of Ethiopia. J EthnobiolEthnomed. 2013;9(1):65. doi:10.1186/1746-4269-9-65

Teklay A. Traditional medicinal plants for ethnoveterinary medicine used in KilteAwulaelo district, Tigray region, Northern Ethiopia. Adv Med Plant Res. 2015;3(4):137–150.

Mekuanent T, Zebene A, Solomon Z. Ethnobotanical study of medicinal plants in Chilga district, Northwestern Ethiopia. J Nat Remedies. 2015;15(2):88–112. doi:10.18311/jnr/2015/476

Mukazayire M-J, Minani V, Ruffo CK, Bizuru E, Stévigny C, Duez P. Traditional phytotherapy remedies used in Southern Rwanda for the treatment of liver diseases. J Ethnopharmacol. 2011;138(2):415–431.doi:10.1016/j.jep.2011.09.025

Pascaline J, Charles M, Lukhoba C, George O. Phytochemical constituents of some medicinal plants used by the Nandis of South Nandi district, Kenya. J Anim Plant Sci. 2011;9(3):1201–1210.

Muthaura C, Rukunga G, Chhabra S, et al. Antimalarial activity of some plants traditionally used in Meru district of Kenya. Phytother Res. 2007;21(9):860–867. doi:10.1002/ptr.2170

Feyera T, Terefe G, Shibeshi W. Evaluation of in vivo antitrypanosomal activity of crude extracts of Artemisia abyssinica against a Trypanosomacongolense isolate. BMC Complement Altern Med.2014;14(1):117. doi:10.1186/1472-6882-14-11

DixitSP,TewariPV,GuptaRM,Experimentalstudiesontheimmunologicalaspects of Atibala(Abutilon indicumLinn), Mahabala (SidarhombifoliaLinn.),Bala(SidacordifoliaLinn.) andBhumibala(SidaveronicaefoliaLam). JRes Indian Med YogaHomeopathy1978; 13(3):50-66.

De Farias Freire SM, Da Silva JAE, LapaAJ, Souccar C, Torres LMB. Analgesicandanti-inflammatorypropertiesofWedeliatrilobatainrodents. Phytother. Res. 1993; 7: 408-414.

ChandanBK et al.Hepatoprotectiveactivity of Woodfordia fructose Kurz flowers against carbon tetrachloride induced hepatotoxicity.J. Ethanopharmacol.2008; 119:218-224.

RaoBG, RaoYV, RaoTM. Hepato protective activity of SpillanthesacmellaExtractsagainstCCl4inducedlivertoxicityinrats.AsianPacJ. Trop. Diseases.2012; S208-S211.

GuptaA, NagariyaAK,MishraAK,BansalP,KumarS,GuptaV, SinghAK, Ethno-potential of medicinal herbs in Liver diseases: An overview Journal of PharmacyResearch2010;3(3): 435-441.

Elaut G, Henkens T, Papeleu P, et al. Molecular mechanisms underlying the dedifferentiation process of isolated hepatocytes and their cultures. Curr Drug Metab. 2006;7(6):629–660. doi:10.2174/138920006778017759

Vargas-Mendoza N, Madrigal-Santillán E, Morales-González Á, et al. Hepatoprotective effect of silymarin. World J Hepatol. 2014;6(3):144. doi:10.4254/wjh.v6.i3.144



How to Cite

Mantry Piyush, and Dr. Jat K. Rakesh. “ANTIOXIDANT POTENTIAL OF VARIOUS EXTRACTS OF COCCINA INDICA, SIDA CORDATA, MEDICAGO SATIVA AND WEDELIA TRILOBATA USING DPPH METHOD”. Tropical Journal of Pharmaceutical and Life Sciences, vol. 9, no. 6, Dec. 2022, pp. 21-27, doi:10.61280/tjpls.v9i6.111.
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