DEVELOPMENT AND VALIDATION OF THE UV-SPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF TETRAHYDROCURCUMIN
DOI:
https://doi.org/10.61280/tjpls.v10i3.124Keywords:
Quantitative determination, UV-spectrophotometric method, UV, ValidationAbstract
The main objective is to develop and validate the UV-spectrophotometric method for the estimation of Tetrahydrocurcumin as per ICH guidelines.
Materials and Methods: A simple, rapid, accurate, and economical UV-spectrophotometric method has been developed for the estimation of tetrahydrocurcumin.
Results: Theλ max of tetrahydrocurcumin was found to be 280nm. The drug follows linearity in the concentration range 2-12 µg mL-1 with a correlation coefficient value of 0.9975. The accuracy of the method was checked by recovery experiment performed at three different levels, i.e., 50%,100% and 120%. The % recovery was found to be in the ranged between 97.81-101.39 with < 1 %RSD, thereby indicating the low variability and a strong agreement between experimental and true values. Ruggedness of the proposed method was studied with the help of two analysts.
Conclusion: The above method was a rapid tool for routine analysis of tetrahydrocurcumin.
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Alvarez-Ricardo Y, Meza-Morales W, Obregón-Mendoza MA, Toscano RA, Núñez-Zarur F, Germán-Acacio JM, Puentes-Díaz N, Alí-Torres J, Arenaza-Corona A, Ramírez-Apan MT, Morales-Morales D. Synthesis, characterization, theoretical studies and antioxidant and cytotoxic evaluation of a series of Tetrahydrocurcumin (THC)-benzylated derivatives. Journal of Molecular Structure. 2023 Feb 5;1273:134355.
Trivedi MK, Panda P, Sethi KK, Gangwar M, Mondal SC, Jana S. Solid and liquid state characterization of tetrahydrocurcumin using XRPD, FT-IR, DSC, TGA, LC-MS, GC-MS, and NMR and its biological activities. Journal of pharmaceutical analysis. 2020 Aug 1;10(4):334-45.
Kakkar V, Kaur IP, Kaur AP, Saini K, Singh KK. Topical delivery of tetrahydrocurcumin lipid nanoparticles effectively inhibits skin inflammation: in vitro and in vivo study. Drug development and industrial pharmacy. 2018 Oct 3;44(10):1701-12.
Sharma JB, Bhatt S, Saini V, Kumar M. Development and validation of uv-visible spectrophotometric method for the estimation of curcumin and tetrahydrocurcumin in simulated intestinal fluid. Research Journal of Pharmacy and Technology. 2021;14(6):2971-5.
Zhang ZB, Luo DD, Xie JH, Xian YF, Lai ZQ, Liu YH, Liu WH, Chen JN, Lai XP, Lin ZX, Su ZR. Curcumin’s metabolites, tetrahydrocurcumin and octahydrocurcumin, possess superior anti-inflammatory effects in vivo through suppression of TAK1-NF-κB pathway. Frontiers in pharmacology. 2018 Oct 17;9:1181.
Panda SK, Nirvanashetty S, Missamma M, Jackson-Michel S. The enhanced bioavailability of free curcumin and bioactive-metabolite tetrahydrocurcumin from a dispersible, oleoresin-based turmeric formulation. Medicine. 2021 Jul 7;100(27).
Kakkar V, Kaur IP, Kaur AP, Saini K, Singh KK. Topical delivery of tetrahydrocurcumin lipid nanoparticles effectively inhibits skin inflammation: in vitro and in vivo study. Drug development and industrial pharmacy. 2018 Oct 3;44(10):1701-12.
Zhang ZB, Luo DD, Xie JH, Xian YF, Lai ZQ, Liu YH, Liu WH, Chen JN, Lai XP, Lin ZX, Su ZR. Curcumin’s metabolites, tetrahydrocurcumin and octahydrocurcumin, possess superior anti-inflammatory effects in vivo through suppression of TAK1-NF-κB pathway. Frontiers in pharmacology. 2018 Oct 17;9:1181.
Godin B, Touitou E. Ethosomes: new prospects in transdermal delivery. Critical Reviews™ in Therapeutic Drug Carrier Systems. 2003;20(1).
Paiva-Santos AC, Silva AL, Guerra C, Peixoto D, Pereira-Silva M, Zeinali M, Mascarenhas-Melo F, Castro R, Veiga F. Ethosomes as nanocarriers for the development of skin delivery formulations. Pharmaceutical research. 2021 Jun;38(6):947-70.
Garg V, Singh H, Bimbrawh S, Kumar Singh S, Gulati M, Vaidya Y, Kaur P. Ethosomes and transfersomes: Principles, perspectives and practices. Current drug delivery. 2017 Aug 1;14(5):613-33.
Dinçer Z, Basan H, Göger NG. Quantitative determination of ambroxol in tablets by derivative UV spectrophotometric method and HPLC. Journal of pharmaceutical and biomedical analysis. 2003 Apr 1;31(5):867-72.
Altria KD, Rudd DR. An overview of method validation and system suitability aspects in capillary electrophoresis. Chromatographia. 1995 Sep;41:325-31.
Hyllbrant B, Tyrefors N, Markides KE, Långström B. On the use of liquid chromatography with radio-and ultraviolet absorbance detection coupled to mass spectrometry for improved sensitivity and selectivity in determination of specific radioactivity of radiopharmaceuticals. Journal of pharmaceutical and biomedical analysis. 1999 Jul 1;20(3):493-501.
Cobb Z, Shaw PN, Lloyd LL, Wrench N, Barrett DA. Evaporative light‐scattering detection coupled to microcolumn liquid chromatography for the analysis of underivatized amino acids: Sensitivity, linearity of response and comparisons with UV absorbance detection. Journal of Microcolumn Separations. 2001;13(4):169-75.
Zhu L, Lee CS, DeVoe DL. Integrated microfluidic UV absorbance detector with attomol-level sensitivity for BSA. Lab on a Chip. 2006;6(1):115-20.
Eaton A. Measuring UV‐absorbing organics: a standard method. Journal‐American Water Works Association. 1995 Feb;87(2):86-90.
Gil M, Escolar D, Iza N, Montero JL. Accuracy and linearity in UV spectrophotometry with a liquid absorbance standard. Applied spectroscopy. 1986 Nov 1;40(8):1156-61
Mulholland M. Ruggedness testing in analytical chemistry. TrAC Trends in Analytical Chemistry. 1988 Nov 1;7(10):383-9.
Biter AB, Pollet J, Chen WH, Strych U, Hotez PJ, Bottazzi ME. A method to probe protein structure from UV absorbance spectra. Analytical biochemistry. 2019 Dec 15;587:113450..
Das SK, Chakraborty S, Roy C, Rajabalaya R, Mohaimin AW, Khanam J, Nanda A, David SR. Ethosomes as novel vesicular carrier: An overview of the principle, preparation and its applications. Current drug delivery. 2018 Jul 1;15(6):795-817.
Mbah CC, Builders PF, Attama AA. Nanovesicular carriers as alternative drug delivery systems: ethosomes in focus. Expert opinion on drug delivery. 2014 Jan 1;11(1):45-59.
Song X, Zhao Y, Hou S, Xu F, Zhao R, He J, Cai Z, Li Y, Chen Q. Dual agents loaded PLGA nanoparticles: systematic study of particle size and drug entrapment efficiency. European journal of pharmaceutics and biopharmaceutics. 2008 Jun 1;69(2):445-53.
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