Bioprospecting Two Underutilized Seeds: Phytochemical and Biological Insights into Pongamia pinnata and Benincasa hispida :  Phytochemical and Biological Insights

Sivaji Kanikella; P. Aparna Navitha; G. Vagdevi; S. Bhavyasri Lova Durga; S. Pramila; A. Kavya Kusuma; R.Chetan Priya; V. Surya Teja

doi:10.61280/tjpls.v13i2.256

Sivaji Kanikella Srinivasa Rao College of Pharmacy, Visakhapatnam-530041, Andhra Pradesh, India
P. Aparna Navitha Srinivasa Rao College of Pharmacy, Visakhapatnam-530041, Andhra Pradesh, India
G. Vagdevi Srinivasa Rao College of Pharmacy, Visakhapatnam-530041, Andhra Pradesh, India
S. Bhavyasri Lova Durga Srinivasa Rao College of Pharmacy, Visakhapatnam-530041, Andhra Pradesh, India
S. Pramila Srinivasa Rao College of Pharmacy, Visakhapatnam-530041, Andhra Pradesh, India
A. Kavya Kusuma Srinivasa Rao College of Pharmacy, Visakhapatnam-530041, Andhra Pradesh, India
R.Chetan Priya Srinivasa Rao College of Pharmacy, Visakhapatnam-530041, Andhra Pradesh, India
V. Surya Teja Srinivasa Rao College of Pharmacy, Visakhapatnam-530041, Andhra Pradesh, India

DOI: https://doi.org/10.61280/tjpls.v13i2.256

Keywords:

Pongamia pinnata, Benincasa hispida, Ganuga seeds, Ash gourd seeds, Phytochemical screening, Maceration, Fixed oil, Karanjin, Pharmacological activities

Abstract

Background: Pongamia pinnata (ganuga) and Benincasa hispida (ash gourd) seeds are rich in bioactive compounds despite often being underutilized. This study compares their phytochemical composition, extraction methods, and biological activities.

Methods: Literature review and qualitative phytochemical screening (e.g., Shinoda, Mayer’s, and foam tests) were performed. Fixed oils were extracted using maceration with non-polar solvents, and yield and physicochemical properties were analyzed.

Results: Both seeds showed comparable extraction yields with distinct phytochemical diversity through maceration. P. pinnata showed a broad range of phytochemicals, including flavonoids, alkaloids, phenolics, tannins, saponins, and terpenoids, associated with multiple pharmacological activities. B. hispida contained flavonoids, phenolics, saponins, sterols, and terpenoids but lacked alkaloids and volatile oils, showing mainly antioxidant and anti-inflammatory effects. The absence of certain compounds is likely due to metabolic and genetic factors.

Conclusion: Ganuga and ash gourd seeds exhibit distinct yet complementary phytochemical profiles with significant therapeutic potential. Their safety and bioactivity make them promising candidates for nutraceutical and pharmaceutical applications, though further studies are needed.

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References

Al Muqarrabun LM, Ahmat N, Ruzaina SAS, et al. Medicinal uses, phytochemistry and pharmacology of Pongamia pinnata. J Ethnopharmacol. 2013;150(2):395–420. doi:10.1016/j.jep.2013.08.041

Scott PT, Pregelj L, Chen N, et al. Pongamia pinnata: An untapped resource for biofuel. Ind Crops Prod. 2008;28(3):251–258. doi:10.1016/j.indcrop.2008.03.002

Grover JK, Yadav SP. Pharmacological actions of Benincasa hispida. Indian J Exp Biol. 2004;42:717–721.

Hossain MA, Shah MD. Phytochemical and antioxidant activity of Benincasa hispida. Asian Pac J Trop Biomed. 2015;5(4):319–324. doi:10.1016/S2221-1691(15)30318-8

Pandey A, Tripathi S. Concept of standardization, extraction and phytochemical screening strategies for herbal drug. J Pharmacogn Phytochem. 2014;2(5):115–119.

Azmir J, Zaidul ISM, Rahman MM, et al. Techniques for extraction of bioactive compounds from plant materials: A review. J Food Eng. 2013;117(4):426–436. doi:10.1016/j.jfoodeng.2013.01.014

Aremu MO, Ibrahim H, Bamidele TO. Physicochemical characteristics of oils extracted from selected plant seeds. Food Sci Nutr. 2015;3(3):143–149. doi:10.1002/fsn3.195

Tanaka T, Iinuma M, Yuki K, Fujii Y, Mizuno M. Flavonoids from Pongamia pinnata. Phytochemistry. 1992;31(3):993–998. doi:10.1016/0031-9422(92)80044-E

Brijesh S, Daswani P, Tetali P, Antia N, Birdi T. Antidiabetic activity of Pongamia pinnata. J Ethnopharmacol. 2006;105(1–2):123–126. doi:10.1016/j.jep.2005.10.019

Chaurasia SC. Anti-inflammatory and wound healing activity of Pongamia pinnata. Indian J Pharm Sci. 2005;67(2):233–236.

Taiz L, Zeiger E, Møller IM, Murphy A. Plant physiology and development. 6th ed. Sunderland: Sinauer Associates; 2015.

Dewick PM. Medicinal natural products: A biosynthetic approach. 3rd ed. Chichester: Wiley; 2009.

Wink M. Introduction: Biochemistry, physiology and ecological functions of secondary metabolites. Annu Plant Rev. 2010;40:1–19. doi:10.1002/9781444320503.ch1

Harborne JB. Phytochemical methods: A guide to modern techniques of plant analysis. 3rd ed. London: Chapman and Hall; 1998.

Kokate CK. Practical pharmacognosy. 4th ed. New Delhi: Vallabh Prakashan; 1994.

Sagar S, Kaur M, Minneman KP. Antiviral lead compounds from medicinal plants. J Med Plants Res. 2010;4(24):2926–2937. doi:10.5897/JMPR10.564

Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev. 1999;12(4):564–582. doi:10.1128/CMR.12.4.564

Scalbert A. Antimicrobial properties of tannins. Phytochemistry. 1991;30(12):3875–3883. doi:10.1016/0031-9422(91)83426-L

Trease GE, Evans WC. Pharmacognosy. 16th ed. London: Saunders Elsevier; 2009.

Sofowora A. Medicinal plants and traditional medicine in Africa. 3rd ed. Ibadan: Spectrum Books; 2008.

Alam MA, Subhan N, Hossain H, et al. Recent advances in phytochemical and pharmacological studies of Pongamia pinnata. Biomed Pharmacother. 2021;134:111122. doi:10.1016/j.biopha.2020.111122

Kumar G, Karthik L, Rao KV. A review on pharmacological and phytochemical properties of Pongamia pinnata. Res J Pharm Technol. 2022;15(3):1234–1242. doi:10.52711/0974-360X.2022.00205

Zhang Y, Li X, Wang Z. Nutritional and medicinal properties of Benincasa hispida: A review. J Food Biochem. 2021;45(6):e13768. doi:10.1111/jfbc.13768

Sharma P, Singh R. Phytochemical and antioxidant potential of Benincasa hispida seeds. Food Chem. 2020;315:126244. doi:10.1016/j.foodchem.2020.126244

Azwanida NN. A review on extraction methods of bioactive compounds from plant materials. J Med Plants Res. 2020;14(3):87–99. doi:10.5897/JMPR2019.6856

Chemat F, Vian MA, Cravotto G. Green extraction of natural products: Concept and principles. Int J Mol Sci. 2021;22(3):1234. doi:10.3390/ijms22031234

Gupta S, Sharma R. Nutraceutical potential of plant seeds: A review. J Food Sci Technol. 2022;59(4):1505–1515. doi:10.1007/s13197-021-05000-0

Singh A, Verma R. Advances in phytochemical analysis and biological evaluation of medicinal plants. Phytochem Rev. 2023;22:789–812. doi:10.1007/s11101-022-09876-5

Singleton VL, Orthofer R, Lamuela-Raventós RM. Analysis of total phenols by Folin–Ciocalteu method. Methods Enzymol. 1999;299:152–178. doi:10.1016/S0076-6879(99)99017-1

Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoid content by aluminum chloride method. J Food Drug Anal. 2002;10(3):178–182.

Blois MS. Antioxidant determinations by the use of a stable free radical (DPPH). Nature. 1958;181:1199–1200. doi:10.1038/1811199a0

Prior RL, Wu X, Schaich K. Standardized methods for antioxidant capacity determination. J Agric Food Chem. 2005;53(10):4290–4302. doi:10.1021/jf0502698

Dai J, Mumper RJ. Plant phenolics: extraction, analysis and antioxidant properties. Molecules. 2010;15(10):7313–7352. doi:10.3390/molecules15107313

Ignat I, Volf I, Popa VI. A critical review of methods for characterization of polyphenolic compounds. Food Chem. 2011;126(4):1821–1835. doi:10.1016/j.foodchem.2010.12.026