HPLC METHOD DEVELOPMENT FOR ESTIMATION GALLIC ACID AND ELLAGIC ACID IN AN AYURVEDIC ANTIARTHRITIC FORMULATION SIMHNAD GUGGUL

Abstract

Present study is a work on results of analytical method development for two important bioactive phyto-constituents of a well-known Ayurvedic formulation Simhnad guggul i.e. gallic acid and ellagic acid. TLC method was used for authentication of these constituents in formulation. Then HPLC methods were developed for quantification of gallic acid and ellagic acid in formulation. The optimized mobile phase for gallic acid was Acetonitrile: water (70:30) and 3% Glacial acetic acid and for ellagic acid it was Acetonitrile: water (80:20) and 1% Glacial acetic acid. The method was applied on market formulations and freshly prepared one. The results indicate that developed methods were found to be precise, sensitive, reliable and accurate.